deaD [2017-05-24 09:35:32]

DEAD-box RNA helicase, important for adaptation to low temperatures

Locus: BSU39110

Isoelectric point: 7.71

Molecular weight: 53.85 kDa

Protein length: 479 aa

Gene length: 1437 bp

Function: RNA helicase

Product: DEAD-box RNA helicase

Essential: no

Synonyms: yxiN

Genomic Context

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Categories containing this gene/protein

• Information processing → RNA synthesis and degradation → DEAD-box RNA helicases
• Information processing → Protein synthesis, modification and degradation → Translation → Translation/ other

Gene

Coordinates: 4,015,987 → 4,017,426

The protein

Catalyzed reaction/ biological activity

• RNA helicase
• required for ribosome assembly PubMed

Protein family

• [DEAD-box RNA helicases|search|DEAD-box RNA helicase]

Paralogous protein(s)

• CshA, CshB, YfmL

Domains

• Helicase domain 1: AS 1-203
• Linker region: AS 204-212
• Helicase domain 2: AS 207-368
• RNA-binding domain: AS 404-479

Structure

• 2G0C (RNA-binding domain, AA 404-479), 2HJV (second domain, AA 207-368); 3MOJ (RNA binding domain complexed with a fragment of 23S ribosomal RNA) PubMed

Expression and Regulation

Operons

• Genes: yxiM-deaD
  Description: PubMed

  Regulation

  • expression declines in the stationary phase PubMed

Biological materials

Mutant

• MGNA-B720 (deaD::erm), available at the NBRP B. subtilis, Japan
• GP1052 (tet), available in the Stülke lab

Expression vector

• for expression/ purification from B. subtilis with C-terminal Strep-tag, for SPINE, expression from the native chromomsomal site: GP1065 (spc), available in Jörg Stülke's lab

Two-hybrid system

• B. pertussis adenylate cyclase-based bacterial two hybrid system (BACTH), available in Jörg Stülke's lab

FLAG-tag construct

• GP1068 (spc, based on pGP1331), available in Jörg Stülke's lab

Labs working on this gene/protein

• Dagmar Klostermeier, Biozentrum Basel, Switzerland homepage

References

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